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pSilencer4.1-CMV neo 构建费用500元/个起

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基本信息

载体类型: 

RNAi系统干扰载体 

载体抗性: 

Amp 

载体标签: 

/ 

筛选标记: 

Neo/G418 

启动子: 

CMV 

表达水平: 

高拷贝 

载体大小: 

4944 bp 

克隆方法: 

多克隆位点,限制性内切酶 

5' 测序引物: 

/ 

3' 测序引物 

/ 

备注:

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质粒图谱和多克隆位点信息

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载体序列

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa

      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttact accgttgtta

      481 taggtgtctc ttgaacacct ataacaacgg tagtggatcc acggttcact aaaccagctc

      541 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt

      601 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt

      661 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt

      721 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag

      781 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat

      841 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag

      901 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg

      961 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc

     1021 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg

     1081 taattgatta ctattaataa ctaagatctg gtaccttgaa ttcatgcttc tcctcccttt

     1141 agtgagggta attctctctc tctccctata gtgagtcgta ttaattcctt ctcttctata

     1201 gtgtcaccta aatcgttgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt

     1261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt

     1321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg

     1381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg

     1441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg

     1501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat

     1561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc

     1621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc

     1681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga

     1741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt

     1801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg

     1861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc

     1921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg

     1981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc

     2041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg

     2101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa aaaaaccacc

     2161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct

     2221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt

     2281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa

     2341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa

     2401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc

     2461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct

     2521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca

     2581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt

     2641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt

     2701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc

     2761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc

     2821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt

     2881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact

     2941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc

     3001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt

     3061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg

     3121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct

     3181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa

     3241 tgttgaatac tcatactctt cctttttcaa tattcagaca tgataagata cattgatgag

     3301 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat

     3361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa

     3421 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc

     3481 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg

     3541 cgtcgcttgg tcggtcattt cgcgaacccc agagtcccgc tcagaagaac tcgtcaagaa

     3601 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc

     3661 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct

     3721 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt

     3781 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg

     3841 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt

     3901 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat

     3961 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg

     4021 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc

     4081 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag

     4141 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt

     4201 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca

     4261 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata

     4321 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa

     4381 acgatcctca tcctgtctct tgatcagatc cgaaaatgga tatacaagct cccgggagct

     4441 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg

     4501 cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat

     4561 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg

     4621 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc

     4681 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc

     4741 ctggggactt tccacaccaa tattattgaa gcatttatca gggttattgt ctcatgagcg

     4801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc

     4861 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata

     4921 ggcgtatcac gaggcccttt cgtc

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