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基本信息
载体类型: | 哺乳动物细胞表达载体 |
载体抗性: | Kan |
载体标签: | Flag |
启动子: | CMV |
筛选标记: | Neo |
表达水平: | 高拷贝 |
载体大小: | 4325bp |
克隆方法: | 多克隆位点,限制性内切酶 |
5' 测序引物: | / |
3' 测序引物: | / |
备注: | 如有侵权请产权持有人尽快联系法务15927229196 |
质粒图谱和多克隆位点信息
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载体序列
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagaagcc cgggcggatc
721 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
781 cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg
841 tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga
901 tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
961 ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa
1021 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
1081 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
1141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1201 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
1801 taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac cagctgtgga
1861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
2041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
2101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
2161 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
2221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
2401 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
2461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
2521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
2581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
2641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
2701 gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
2761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
2821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
2881 caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga atgggctgac
2941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
3121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
3181 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
3241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
3301 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
3361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
3421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
3481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
3541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
3601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
3661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
3721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
3781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
3841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
3901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
3961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4321 ccgcc
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