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基本信息  

载体类型：	RNAi系统干扰载体
载体抗性：	Kan
载体标签：	EGFP
启动子：	U6
筛选标记：	Neo
表达水平:	高拷贝
载体大小:	4857bp
克隆方法:	多克隆位点，限制性内切酶
5' 测序引物:	/
3' 测序引物：	/

质粒图谱和多克隆位点信息

注意：

客户在我公司选择构建载体服务项目时，请自行提供母源载体。

如要使用本公司保存的母源载体时，本公司不能保证所有母源载体完全符合客户预期，也不承担由此产生的任何责任。

  

载体描述

pGenesil-1质粒是以pEGFP-C1质粒为骨架改造获得的。pEGFP-C1质粒广泛用于哺乳动物细胞已经多年，它具有荧光蛋白表达，非常方便导入效果的检测。

（1） 调控shRNA的启动子为hU6启动子。shRNA插入位点：BamHI、HindIII; 

（2） 此载体带有EGFP荧光基因，可作为转染指示; 

（3） 此载体带有Neo抗性基因，可在真核细胞中用G418进行筛选。

载体序列

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc

      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac

     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag

     1321 ctgtacaagt ccggactcag atctcgagat ccaccggatc tagataactg atcataatca

     1381 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga

     1441 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg

     1501 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt

     1561 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtcccggga agcttgcatg

     1621 cctgcaggtc gactctagag gatcccgcgt cctttccaca agatatataa acccaagaaa

     1681 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa

     1741 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt

     1801 gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa

     1861 tatgaaggaa tcatgggaaa taggccctct tcctgcccga ccttggcgcg cgctcggcgc

     1921 gcggtcacgc tccgtcacgt ggtgcgtttt gcctgcgcgt ctttccactg ggggaattcg

     1981 tcgactctag acactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg

     2041 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc

     2101 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc

     2161 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca

     2221 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt

     2281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata

     2341 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta

     2401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat

     2461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc

     2521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta

     2581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca

     2641 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga

     2701 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga

     2761 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga

     2821 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg

     2881 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga

     2941 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt

     3001 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct

     3061 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct

     3121 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg

     3181 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca

     3241 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga

     3301 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt

     3361 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt

     3421 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct

     3481 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt

     3541 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca

     3601 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg

     3661 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct

     3721 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg

     3781 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt

     3841 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc

     3901 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca

     3961 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt

     4021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc

     4081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag

     4141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa

     4201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg

     4261 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag

     4321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg

     4381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga

     4441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc

     4501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc

     4561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga

     4621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt

     4681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg

     4741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac

     4801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat

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