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基本信息
| 载体类型： | 慢病毒表达质粒；microRNA表达质粒。 |
| 载体抗性： | Amp |
| 载体标签： | ZsGreen1 |
| 启动子： | CMV IE promoter |
| 表达水平: | 高拷贝 |
| 载体大小: | 8250 bp |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 5' 测序引物: | / |
| 3' 测序引物： | 5’-AATAGGCGTATCACGAGG-3’ |
| 备注： | 如有侵权请产权持有人尽快联系法务15927229196 |
质粒图谱和多克隆位点信息
注意：
客户在我公司选择构建载体服务项目时，请自行提供母源载体。
如要使用本公司保存的母源载体时，本公司不能保证所有母源载体完全符合客户预期，也不承担由此产生的任何责任。
载体描述
pHAGE-CMV-MCS-IZsGreen为慢病毒表达质粒；microRNA表达质粒。
载体序列
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc catgttccat
2821 gtttctttta ggtatatctt tggacttcct cccctgatcc ttgttctgtt gccagtagca
2881 tcatctgatt gtgatattga aggtaaagat ggcaaacaat atgagagtgt tctaatggtc
2941 agcatcgatc aattattgga cagcatgaaa gaaattggta gcaattgcct gaataatgaa
3001 tttaactttt ttaaaagaca tatctgtgat gctaataagg aaggtatgtt tttattccgt
3061 gctgctcgca agttgaggca atttcttaaa atgaatagca ctggtgattt tgatctccac
3121 ttattaaaag tttcagaagg cacaacaata ctgttgaact gcactggcca ggttaaagga
3181 agaaaaccag ctgccctggg tgaagcccaa ccaacaaaga gtttggaaga aaataaatct
3241 ttaaaggaac agaaaaaact gaatgacttg tgtttcctaa agagactatt acaagagata
3301 aaaacttgtt ggaataaaat tttgatgggc actaaagaac actaaaggat ccctcccccc
3361 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
3421 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
3481 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
3541 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
3601 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
3661 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
3721 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
3781 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
3841 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
3901 gaaaaacacg atgataatat ggccacacat atggcccagt ccaagcacgg cctgaccaag
3961 gagatgacca tgaagtaccg catggagggc tgcgtggacg gccacaagtt cgtgatcacc
4021 ggcgagggca tcggctaccc cttcaagggc aagcaggcca tcaacctgtg cgtggtggag
4081 ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc
4141 gtgttcaccg agtaccccca ggacatcgtc gactacttca agaactcctg ccccgccggc
4201 tacacctggg accgctcctt cctgttcgag gacggcgccg tgtgcatctg caacgccgac
4261 atcaccgtga gcgtggagga gaactgcatg taccacgagt ccaagttcta cggcgtgaac
4321 ttccccgccg acggccccgt gatgaagaag atgaccgaca actgggagcc ctcctgcgag
4381 aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg acgtgagcat gtacctgctg
4441 ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg
4501 ccccgcaaga tgcccgactg gcacttcatc cagcacaagc tgacccgcga ggaccgcagc
4561 gacgccaaga accagaagtg gcacctgacc gagcacgcca tcgcctccgg ctccgccttg
4621 ccctgaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
4681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
4741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
4801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
4861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
4921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
4981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
5041 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
5101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
5161 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
5221 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
5281 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
5341 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
5401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
5461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
5521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
5581 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
5641 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
5701 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
5761 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
5821 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5881 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5941 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
6001 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
6061 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
6121 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
6181 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
6241 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
6301 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
6361 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
6421 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
6481 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
6541 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
6601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
6661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
6781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
7021 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
7081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
7141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
7201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
7261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
7321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
7381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
7441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
7501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
7561 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
7621 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
7681 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
7741 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
7801 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
7861 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
7921 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
7981 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
8041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
8101 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
8161 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
8221 ttgttaaata tgtactacaa acttagtagt
//
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