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基本信息
载体类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体;鱼类表达载体 |
载体抗性: | Amp |
载体标签: | HA |
启动子: | simian CMV IE94、lac、T3、SP6 |
高拷贝/低拷贝: | 高拷贝 |
载体大小: | 4113 bp |
克隆方法: | 多克隆位点,限制性内切酶 |
5' 测序引物: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
备注: | 如有侵权请产权持有人尽快联系法务15927229196 |
质粒图谱和多克隆位点信息
载体描述
pCS2+载体能够在多种细胞中进行高水平的瞬表达。表达细胞:原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。pCS2+载体同时也能进行体外转录和翻译。本载体在pCS2+载体基础上改造而来,增加了HA蛋白标签,便于检测。
载体序列
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattca tgtacccata cgacgtccca
121 gactacgctt aatctagaac tatagtgagt cgtattacgt agatccagac atgataagat
181 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
241 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
301 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
361 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg
421 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
1321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
1561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
1621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
1681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
1741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
1801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
1861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
1921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
1981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
2041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
2101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
2161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
2221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
2281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
2341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
2401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
2461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
2521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
2581 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
2641 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
2701 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
2761 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
2821 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
2881 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
2941 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
3001 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
3061 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
3121 cgccagtcga ccatagccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg
3181 tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg
3241 tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg
3301 cggacttggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga
3361 ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg
3421 ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa
3481 tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag tattccatat
3541 atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat
3601 ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg
3661 gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg
3721 acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc
3781 caccattgac gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat
3841 tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact
3901 tggcaagtac attactattg gaaggacgcc agggtacatt ggcagtactc ccattgacgt
3961 caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga
4021 ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg
4081 gaggtctata taagcaatgc tcgtttaggg aac
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