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基本信息

质粒图谱和多克隆位点信息

注意：

客户在我公司选择构建载体服务项目时，请自行提供母源载体。

如要使用本公司保存的母源载体时，本公司不能保证所有母源载体完全符合客户预期，也不承担由此产生的任何责任。

载体描述

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.