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基本信息
载体类型： 酵母单杂交载体 载体抗性： Kan 载体标签： / 筛选标记： TRP1，HIS3 启动子： TRP1, T7, T3 表达水平: 高拷贝 载体大小: 7.2kb 克隆方法: 多克隆位点，限制性内切酶 培养条件: 培养条件: 37℃，有氧 LB 表达宿主: 酵母细胞 5' 测序引物: M13F:5’-TGTAAAACGACGGCCAGT-3’ 3' 测序引物： -- 备注 如有侵权请产权持有人尽快联系法务15927229196
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载体序列
ORIGIN
1 gaattcccgg ggagctcacg cgttcgcgaa tcgatccgcg gtctagaaat tcctggcatt
61 atcacataat gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa
121 aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg
181 tattacaaat gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat
241 agagcactcg atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc
301 gcaagtgatt aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc
361 caagcattcc ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca
421 tcacaccact gaagactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc
481 cgtgcgtgga gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag
541 agcggtggta gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga
601 gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc
661 tagcagaatt accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga
721 gagtgcgttc aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa
781 cgatgttccc tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc
841 atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg
901 aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg
961 aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgagaaaa
1021 aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg tggcaagtgg
1081 tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag cgaacaagtg
1141 caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca cttaagagga
1201 aaaccaagaa gtgccagaaa taacatgagc aactacaata acaacaacgg cggctacaac
1261 ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta cggcaacggt
1321 ggtttcttcg gtggaaacaa cggtggcagc agatctaacg gccgttctgg tggtagatgg
1381 atcgatggca aacatgtccc agctccaaga aacgaaaagg ccgagatcgc catatttggt
1441 gtccccgagg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
1501 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
1561 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
1621 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
1681 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt
1741 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
1801 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
1861 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
1921 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
1981 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
2041 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
2101 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
2161 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat
2221 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg
2281 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
2341 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
2401 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc
2461 atcgcgtccg ccatctccag cagccgcacg cggcgcatcg gggggggggt ttcaattcaa
2521 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttaacga
2581 cattactata tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac
2641 tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg
2701 tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc
2761 ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg
2821 agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg
2881 tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc
2941 tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat
3001 tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg
3061 cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct
3121 ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata
3181 ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat
3241 actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc
3301 aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga
3361 gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga
3421 tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg
3481 tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac
3541 tgagtagtat ttatttaagt attgtttgtg cacttgccct agagtcgacc tgcaggcatg
3601 caagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc
3661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
3721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
3781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
3841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
3901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
3961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
4141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
4201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
4261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
4321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
4381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
4441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
4501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
4561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
4621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
4681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
4741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
4801 cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
4861 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
4921 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
4981 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
5041 cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
5101 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
5161 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
5221 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
5281 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
5341 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
5401 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
5461 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
5521 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
5581 ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat tgaagcattt
5641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
5701 taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg cttgcctgta
5761 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta
5821 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagagtaga agagttacgg
5881 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat
5941 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta
6001 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc
6061 ggcattaata cctgagagca ggaagagcaa gataaaaggn agtatttgtt ggcgatcccc
6121 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
6181 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
6241 acgtgatgaa aaggaccgac gtctaagaaa ccattattat catgacatta acctataaaa
6301 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
6361 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
6421 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
6481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
6541 taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt
6601 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
6661 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
6721 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
6781 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
6841 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
6901 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
6961 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
7021 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
7081 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
7141 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
7201 atagggc
//
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