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pHIS2 构建费用300元/个起

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基本信息

载体类型:

酵母单杂交载体

载体抗性:

Kan

载体标签

/

筛选标记:

TRP1HIS3

启动子

TRP1, T7, T3

表达水平:

拷贝

载体大小:

7.2kb

克隆方法:

多克隆位点,限制性内切酶

培养条件:

培养条件: 37℃,有氧 LB

表达宿主:

酵母细胞

5' 测序引物:

M13F:5-TGTAAAACGACGGCCAGT-3

3' 测序引物

--

备注

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质粒图谱和多克隆位点信息

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载体序列

ORIGIN

        1 gaattcccgg ggagctcacg cgttcgcgaa tcgatccgcg gtctagaaat tcctggcatt

       61 atcacataat gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa

      121 aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg

      181 tattacaaat gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat

      241 agagcactcg atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc

      301 gcaagtgatt aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc

      361 caagcattcc ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca

      421 tcacaccact gaagactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc

      481 cgtgcgtgga gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag

      541 agcggtggta gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga

      601 gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc

      661 tagcagaatt accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga

      721 gagtgcgttc aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa

      781 cgatgttccc tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc

      841 atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg

      901 aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg

      961 aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgagaaaa

     1021 aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg tggcaagtgg

     1081 tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag cgaacaagtg

     1141 caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca cttaagagga

     1201 aaaccaagaa gtgccagaaa taacatgagc aactacaata acaacaacgg cggctacaac

     1261 ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta cggcaacggt

     1321 ggtttcttcg gtggaaacaa cggtggcagc agatctaacg gccgttctgg tggtagatgg

     1381 atcgatggca aacatgtccc agctccaaga aacgaaaagg ccgagatcgc catatttggt

     1441 gtccccgagg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc

     1501 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg

     1561 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt

     1621 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct

     1681 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt

     1741 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc

     1801 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt

     1861 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt

     1921 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt

     1981 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct

     2041 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg

     2101 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg

     2161 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat

     2221 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg

     2281 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac

     2341 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga

     2401 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc

     2461 atcgcgtccg ccatctccag cagccgcacg cggcgcatcg gggggggggt ttcaattcaa

     2521 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttaacga

     2581 cattactata tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac

     2641 tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg

     2701 tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc

     2761 ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg

     2821 agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg

     2881 tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc

     2941 tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat

     3001 tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg

     3061 cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct

     3121 ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata

     3181 ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat

     3241 actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc

     3301 aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga

     3361 gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga

     3421 tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg

     3481 tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac

     3541 tgagtagtat ttatttaagt attgtttgtg cacttgccct agagtcgacc tgcaggcatg

     3601 caagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc

     3661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca

     3721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct

     3781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac

     3841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc

     3901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt

     3961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg

     4021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg

     4081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat

     4141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta

     4201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct

     4261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc

     4321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa

     4381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg

     4441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag

     4501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt

     4561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta

     4621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc

     4681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca

     4741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa

     4801 cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat

     4861 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt

     4921 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc

     4981 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat

     5041 cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca

     5101 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg

     5161 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg

     5221 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg

     5281 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt

     5341 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca

     5401 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct

     5461 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc

     5521 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac

     5581 ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat tgaagcattt

     5641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa

     5701 taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg cttgcctgta

     5761 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta

     5821 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagagtaga agagttacgg

     5881 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat

     5941 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta

     6001 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc

     6061 ggcattaata cctgagagca ggaagagcaa gataaaaggn agtatttgtt ggcgatcccc

     6121 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac

     6181 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc

     6241 acgtgatgaa aaggaccgac gtctaagaaa ccattattat catgacatta acctataaaa

     6301 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct

     6361 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac

     6421 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg

     6481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg

     6541 taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt

     6601 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta

     6661 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc

     6721 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg

     6781 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact

     6841 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt

     6901 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc

     6961 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc

     7021 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc

     7081 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc

     7141 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact

     7201 atagggc

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