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基本信息
载体类型: 哺乳动物细胞表达载体 载体抗性: Amp 筛选标记: Neomycin / G418 启动子: CMV 载体大小: 5428bp 克隆方法: 多克隆位点,限制性内切酶 5' 测序引物: / 3' 测序引物: / 备注: 如有侵权请产权持有人尽快联系法务15927229196
质粒图谱和多克隆位点信息
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载体序列
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
2101 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
2161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
2221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
2281 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
2341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
2461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
2521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
2581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
2641 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
2701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
2761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
2821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
2881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
2941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
3001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
3061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
3121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
3241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
3301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
3361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
3421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
3481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
4021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
4141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4201 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
4261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
4321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
4381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
5041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5401 atttccccga aaagtgccac ctgacgtc
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