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基本信息
载体类型: | 哺乳动物细胞表达载体;红色荧光蛋白报告载体 ;分泌表达蛋白 |
载体抗性: | Kan |
载体标签: | EGFP,绿色荧光蛋白 |
筛选标记: | 新霉素/G418 |
启动子: | CMV |
表达水平: | 高拷贝 |
载体大小: | 4769bp |
克隆方法: | 多克隆位点,限制性内切酶 |
5' 测序引物: | CMV-F:5’-CGCAAATGGGCGGTAGGCGTG-3’ |
3' 测序引物: | RFP-R:5’-GCCCTCCATGCGCACCTT-3’ |
备注: | 如有侵权请产权持有人尽快联系法务15927229196 |
质粒图谱和多克隆位点信息
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载体序列
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccatgaa aattgtcgct cctgtcaaac aaactcttaa ctttgattta ctcaaactgg
721 ctggggatgt agaaagcaat ccaggtccag ccacaaccat ggcctcctcc gaggacgtca
781 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
901 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
961 gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
1081 cccaggactc ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga
1141 acttcccctc cgacggcccc gtaatgcaga agaagactat gggctgggag gcctccaccg
1201 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga
1261 aggacggcgg ccactacctg gtggagttca agtctatcta tatggccaag aagcccgtgc
1321 agctgcccgg ctactactac gtggactcca agctggacat cacctcccac aacgaggact
1381 acaccatcgt ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgtagcggc
1441 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
1501 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
1561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
1621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
1681 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
1741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
1801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
1861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
1921 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
1981 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
2041 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
2101 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
2161 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
2221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
2281 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2401 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
2461 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
2521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
2581 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
2641 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2701 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
2761 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
2821 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
2881 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
2941 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
3001 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3061 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3121 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3181 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3241 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3301 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3361 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3421 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3481 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3541 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3601 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
3661 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
3721 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
3781 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
3841 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
3901 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
3961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
4021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
4081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
4141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
4201 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
4261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
4321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
4561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
4621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
4681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
4741 ctgtggataa ccgtattacc gccatgcat
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